=================== recursive_dbscan.py =================== .. code-block:: bash usage: recursive_dbscan.py Perform marker gene guided binning of metagenome contigs using annotations (when available) of sequence composition, coverage and homology. optional arguments: -h, --help show this help message and exit --kmers filepath Path to embedded k-mers table (default: None) --coverages filepath Path to metagenome coverages table (default: None) --gc-content filepath Path to metagenome GC contents table (default: None) --markers filepath Path to Autometa annotated markers table (default: None) --output-binning filepath Path to write Autometa binning results (default: None) --output-main filepath Path to write Autometa main table used during/after binning (default: None) --clustering-method {dbscan,hdbscan} Clustering algorithm to use for recursive binning. (default: dbscan) --completeness 0 < float <= 100 completeness cutoff to retain cluster. e.g. cluster completeness >= `completeness` (default: 20.0) --purity 0 < float <= 100 purity cutoff to retain cluster. e.g. cluster purity >= `purity` (default: 95.0) --cov-stddev-limit float coverage standard deviation limit to retain cluster e.g. cluster coverage standard deviation <= `cov- stddev-limit` (default: 25.0) --gc-stddev-limit float GC content standard deviation limit to retain cluster e.g. cluster GC content standard deviation <= `gc- content-stddev-limit` (default: 5.0) --taxonomy filepath Path to Autometa assigned taxonomies table (default: None) --starting-rank {superkingdom,phylum,class,order,family,genus,species} Canonical rank at which to begin subsetting taxonomy (default: superkingdom) --reverse-ranks Reverse order at which to split taxonomy by canonical- rank. When `--reverse-ranks` is given, contigs will be split in order of species, genus, family, order, class, phylum, superkingdom. (default: False) --rank-filter {superkingdom,phylum,class,order,family,genus,species} Taxonomy column canonical rank to subset by provided value of `--rank-name-filter` (default: superkingdom) --rank-name-filter RANK_NAME_FILTER Only retrieve contigs with this name corresponding to `--rank-filter` column (default: bacteria) --verbose log debug information (default: False) --cpus int Number of cores to use by clustering method (default will try to use as many as are available) (default: -1)